MDR1/ABCB1 Antibody (2U4E9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15660

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15660-100ul
NBP3-15660-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2U4E9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MDR1/ABCB1 Antibodies »

Product Summary for MDR1/ABCB1 Antibody (2U4E9)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 393-710 of human MDR1/ABCB1 (P08183). EFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MDR1/ABCB1 Antibody (2U4E9)

Western Blot: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660]

Western Blot: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660]

Western Blot: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660] - Western blot analysis of extracts of various cell lines, using MDR1/ABCB1 antibody (NBP3-15660) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660] - Immunohistochemistry of paraffin-embedded mouse liver using MDR1/ABCB1 Rabbit mAb (NBP3-15660) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660]

Western Blot: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660]

Western Blot: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660] - Western blot analysis of extracts of Rat liver, using MDR1/ABCB1 antibody (NBP3-15660) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
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Applications for MDR1/ABCB1 Antibody (2U4E9)

Application
Recommended Usage

Western Blot

1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MDR1/ABCB1

MDR1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). MDR1 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. (provided by RefSeq)

Long Name

ATP-binding Cassette, Sub-family B (MDR/TAP), Member 1B

Alternate Names

ABC20, ABCB1, ABCB1B, CD243, CLCS, GP170, IBD13, PGY1

Gene Symbol

ABCB1

Additional MDR1/ABCB1 Products

Product Documents for MDR1/ABCB1 Antibody (2U4E9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MDR1/ABCB1 Antibody (2U4E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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