MDR1/ABCB1 Antibody (SN06-42)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67667
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SN06-42
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human MDR1/ABCB1 aa 373-730 / 1,280. (SwissProt: P08183 Human; SwissProt: P06795 Mouse; SwissProt: P43245 Rat)
Localization
Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MDR1/ABCB1 Antibody (SN06-42)
Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]
Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of P Glycoprotein on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded human liver tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.Applications for MDR1/ABCB1 Antibody (SN06-42)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:1000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 31527311).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using NBP2-67667 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MDR1/ABCB1
Long Name
ATP-binding Cassette, Sub-family B (MDR/TAP), Member 1B
Alternate Names
ABC20, ABCB1, ABCB1B, CD243, CLCS, GP170, IBD13, PGY1
Gene Symbol
ABCB1
Additional MDR1/ABCB1 Products
Product Documents for MDR1/ABCB1 Antibody (SN06-42)
Product Specific Notices for MDR1/ABCB1 Antibody (SN06-42)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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