MDR1/ABCB1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-80870

Novus Biologicals, part of Bio-Techne
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NB100-80870
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all MDR1/ABCB1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide from human MDR1/ABCB1.

Epitope

Internal region near amino acids 255-280

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MDR1/ABCB1 Antibody - BSA Free

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody - BSA Free [NB100-80870]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody - BSA Free [NB100-80870]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NB100-80870] - Staining of human kidney tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Applications for MDR1/ABCB1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:10000 - 1:50000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1:200 - 1:2000
Application Notes
IHC: NB100-80870 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-80870 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: MDR1/ABCB1

P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas.

Long Name

ATP-binding Cassette, Sub-family B (MDR/TAP), Member 1B

Alternate Names

ABC20, ABCB1, ABCB1B, CD243, CLCS, GP170, IBD13, PGY1

Entrez Gene IDs

5243 (Human); 18671 (Mouse); 24646 (Rat)

Gene Symbol

ABCB1

UniProt

P08183 (Human)

Additional MDR1/ABCB1 Products

Product Documents for MDR1/ABCB1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MDR1/ABCB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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