MDR1/ABCB1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-17251
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Feline (92%), Rabbit (93%), Rhesus Macaque (93%), Sheep (93%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MDR1/ABCB1. The exact sequence is proprietary.
Reactivity Notes
Cat (92%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
141 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MDR1/ABCB1 Antibody
Immunocytochemistry/ Immunofluorescence: MDR1/ABCB1 Antibody [NBP2-17251]
Immunocytochemistry/Immunofluorescence: MDR1/ABCB1 Antibody [NBP2-17251] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: P Glycoprotein protein stained by P Glycoprotein antibody diluted at 1:500. Blue: Hoechst 33342 staining.Western Blot: MDR1/ABCB1 Antibody [NBP2-17251] -
Western Blot: MDR1/ABCB1 Antibody [NBP2-17251] - Unboiled HepG2 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MDR1/ABCB1 antibody (NBP2-17251) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)Applications for MDR1/ABCB1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MDR1/ABCB1
Long Name
ATP-binding Cassette, Sub-family B (MDR/TAP), Member 1B
Alternate Names
ABC20, ABCB1, ABCB1B, CD243, CLCS, GP170, IBD13, PGY1
Gene Symbol
ABCB1
UniProt
Additional MDR1/ABCB1 Products
Product Documents for MDR1/ABCB1 Antibody
Product Specific Notices for MDR1/ABCB1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.