ME1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86129

Novus Biologicals, part of Bio-Techne
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NBP1-86129
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ME1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKEKAIKKIWLVDSKGLIVKGRASLTQEKEKFAHEHEEMKNLEAIVQEIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAEC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ME1 Antibody

Western Blot: ME1 Antibody [NBP1-86129]

Western Blot: ME1 Antibody [NBP1-86129]

Western Blot: ME1 Antibody [NBP1-86129] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: ME1 Antibody [NBP1-86129]

Immunohistochemistry-Paraffin: ME1 Antibody [NBP1-86129]

Immunohistochemistry-Paraffin: ME1 Antibody [NBP1-86129] - Staining in human adrenal gland and kidney tissues using anti-ME1 antibody. Corresponding ME1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ME1 Antibody [NBP1-86129]

Immunohistochemistry-Paraffin: ME1 Antibody [NBP1-86129]

Immunohistochemistry-Paraffin: ME1 Antibody [NBP1-86129] - Staining of human adrenal gland shows high expression.
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Applications for ME1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ME1

ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Alternate Names

EC 1.1.1, EC 1.1.1.40, HUMNDME, malate dehydrogenase, Malic enzyme 1, malic enzyme 1, NADP(+)-dependent, cytosolic, malic enzyme 1, soluble, Malic enzyme, cytoplasmic, MES, NADP-dependent malic enzyme, NADP-ME, pyruvic-malic carboxylase

Gene Symbol

ME1

Additional ME1 Products

Product Documents for ME1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ME1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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