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ME2 Antibody (S04-7I2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15058

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-15058-100ul
NBP3-15058-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S04-7I2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for ME2 Antibody (S04-7I2)

Immunogen

A synthetic peptide of human ME2 (Uniprot#: P23368)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ME2 Antibody (S04-7I2)

Western Blot: ME2 Antibody (S04-7I2) [NBP3-15058]

Western Blot: ME2 Antibody (S04-7I2) [NBP3-15058]

Western Blot: ME2 Antibody (S04-7I2) [NBP3-15058] - Western blot detection of ME2 in K562, Hela cell lysates using ME2 Rabbit mAb (1:1000 diluted). Predicted band size: 65kDa. Observed band size: 65kDa.
Immunocytochemistry/ Immunofluorescence: ME2 Antibody (S04-7I2) [NBP3-15058]

Immunocytochemistry/ Immunofluorescence: ME2 Antibody (S04-7I2) [NBP3-15058]

Immunocytochemistry/Immunofluorescence: ME2 Antibody (S04-7I2) [NBP3-15058] - Immunofluorescence of ME2 (green) in Hela cells using ME2 Rabbit mAb at dilution 1/50, and DAPI (blue)

Applications for ME2 Antibody (S04-7I2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20-1/50

Western Blot

1/1000-1/5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ME2

The ME2 gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been s

Alternate Names

EC 1.1.1, EC 1.1.1.38, malate dehydrogenase, Malic enzyme 2, malic enzyme 2, NAD(+)-dependent, mitochondrial, NAD-dependent malic enzyme, mitochondrial, NAD-ME, ODS1, pyruvic-malic carboxylase

Gene Symbol

ME2

Additional ME2 Products

Product Documents for ME2 Antibody (S04-7I2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ME2 Antibody (S04-7I2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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