MeCP2 [p Ser421] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29524

Novus Biologicals, part of Bio-Techne
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NBP2-29524
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Rat

Predicted:

Human (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MeCP2 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 conjugated to KLH. Accession # Q9Z2D6

Modification

p Ser421

Specificity

Specific for the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling of the MeCP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.

Marker

Nucleus Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MeCP2 [p Ser421] Antibody

Western Blot: MeCP2 [p Ser421] Antibody [NBP2-29524]

Western Blot: MeCP2 [p Ser421] Antibody [NBP2-29524]

Western Blot: MeCP2 [p Ser421] Antibody [NBP2-29524] - Western blot of mouse whole brain showing specific immunolabeling of the ~55k truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling is greatly reduced by preadsorption with the phospho-peptide used as antigen (peptide), but not by the corresponding dephospho-peptide (not shown).

Applications for MeCP2 [p Ser421] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MeCP2

MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment (Hutchinson et al., 2012).

Alternate Names

AUTSX3, DKFZp686A24160, MeCp-2 protein, mental retardation, X-linked 79, methyl CpG binding protein 2 (Rett syndrome), X-linked 16

Entrez Gene IDs

17257 (Mouse)

Gene Symbol

MECP2

UniProt

Q9Z2D6 (Mouse)

Additional MeCP2 Products

Product Documents for MeCP2 [p Ser421] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MeCP2 [p Ser421] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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