MeCP2 Antibody

Western Blot: MeCP2 Antibody [NBP3-05542]

Western Blot: MeCP2 Antibody [NBP3-05542] - Western blot analysis of tissue and cell lysates using MeCP2 Antibody at a dilution of 1:20,000 in green: [1] protein standard (red), [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain lysate, [6] C6 cell lysate, and [7] SH-SY5Y cell lysate. The strong band at about 75kDa corresponds to the MeCP2 protein. The MeCP2 proteins of rat and human origin are known to migrate slightly differently on SDS-PAGE gels compared to that of mouse, and as a result bands appear at somewhat different apparent molecular weights.

Immunocytochemistry/ Immunofluorescence: MeCP2 Antibody [NBP3-05542]

Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP3-05542] - Immunofluorescent analysis of rat olfactory bulb section stained with chicken pAb to MeCP2 at a dilution of 1:2,000 in red and costained with mouse mAb to alpha-synuclein at a dilution of 1:1,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. The MeCP2 antibody specifically labels the nuclei of neuronal cells while the alpha-synuclein antibody reveals alpha-synuclein protein concentrated in presynaptic regions.