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MED10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13045

Novus Biologicals, part of Bio-Techne
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NBP3-13045

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%), Rhesus Macaque (98%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MED10. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for MED10 Antibody

Western Blot: MED10 Antibody [NBP3-13045]

Western Blot: MED10 Antibody [NBP3-13045]

Western Blot: MED10 Antibody [NBP3-13045] - 293T whole cell and nuclear extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MED10 antibody (NBP3-13045) diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: MED10 Antibody [NBP3-13045]

Immunocytochemistry/ Immunofluorescence: MED10 Antibody [NBP3-13045]

Immunocytochemistry/Immunofluorescence: MED10 Antibody [NBP3-13045] - MED10 antibody detects MED10 protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MED10 protein stained by MED10 antibody (NBP3-13045) diluted at 1:500. Blue: Hoechst 33342 staining.

Applications for MED10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MED10

MED10 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activatetranscription via RNA polymerase II (Sato et al., 2003 (PubMed 12584197)).(supplied by OMIM)

Alternate Names

L6, mediator complex subunit 10TRG-17, mediator of RNA polymerase II transcription subunit 10, mediator of RNA polymerase II transcription, subunit 10, mediator of RNA polymerase II transcription, subunit 10 homolog (NUT2, S.cerevisiae), MGC5309, NUT2TRG-20, Transformation-related gene 17 protein, Transformation-related gene 20 protein, TRG20

Gene Symbol

MED10

Additional MED10 Products

Product Documents for MED10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MED10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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