MED15 Antibody (OTI1C11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45780

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C11

Concentration

1 mg/ml

View all MED15 Antibodies »

Product Summary for MED15 Antibody (OTI1C11)

Immunogen

Human recombinant protein fragment corresponding to amino acids 393-679 of human MED15(NP_056973) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

82.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MED15 Antibody (OTI1C11)

Western Blot: MED15 Antibody (OTI1C11) [NBP2-45780]

Western Blot: MED15 Antibody (OTI1C11) [NBP2-45780]

Western Blot: MED15 Antibody (1C11) [NBP2-45780] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED15.
Immunohistochemistry: MED15 Antibody (OTI1C11) [NBP2-45780]

Immunohistochemistry: MED15 Antibody (OTI1C11) [NBP2-45780]

Immunohistochemistry: MED15 Antibody (1C11) [NBP2-45780] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: MED15 Antibody (OTI1C11) [NBP2-45780]

Immunohistochemistry: MED15 Antibody (OTI1C11) [NBP2-45780]

Immunohistochemistry: MED15 Antibody (1C11) [NBP2-45780] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications for MED15 Antibody (OTI1C11)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MED15

MED15 is encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ARC105Arc105, CAG7A, CTG repeat protein 7a, CTG7A, FLJ42282, FLJ42935, mediator complex subunit 15PC2 glutamine/Q-rich-associated protein, mediator of RNA polymerase II transcription subunit 15, PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associatedprotein, PC2-glutamine-rich-associated protein, PCQAPDKFZp686A2214, Positive cofactor 2 glutamine/Q-rich-associated protein, positive cofactor 2, glutamine/Q-rich-associated protein, TIG1DKFZp762B1216, TIG-1TPA-inducible gene 1 protein, TNRC7, TPA inducible gene-1, TPA inducible protein, trinucleotide repeat containing 7,105-kD, Trinucleotide repeat-containing gene 7 protein

Gene Symbol

MED15

Additional MED15 Products

Product Documents for MED15 Antibody (OTI1C11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MED15 Antibody (OTI1C11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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