MED21 Antibody (6B6)

Novus Biologicals, part of Bio-Techne | Catalog # H00009412-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6B6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MED21 Antibodies »

Product Specifications

Immunogen

SURB7 (AAH08380, 1 a.a. ~ 144 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQLKTRSGTHSQSLPDS

Specificity

SURB7 (6B6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for MED21 Antibody (6B6)

Western Blot: MED21 Antibody (6B6) [H00009412-M03]

Western Blot: MED21 Antibody (6B6) [H00009412-M03]

Western Blot: MED21 Antibody (6B6) [H00009412-M03] - Analysis of SURB7 expression in HeLa (Cat # L013V1).
Immunocytochemistry/ Immunofluorescence: MED21 Antibody (6B6) [H00009412-M03]

Immunocytochemistry/ Immunofluorescence: MED21 Antibody (6B6) [H00009412-M03]

Immunocytochemistry/Immunofluorescence: MED21 Antibody (6B6) [H00009412-M03] - Analysis of monoclonal antibody to SURB7 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: MED21 Antibody (6B6) [H00009412-M03]

ELISA: MED21 Antibody (6B6) [H00009412-M03]

ELISA: MED21 Antibody (6B6) [H00009412-M03] - Detection limit for recombinant GST tagged SURB7 is approximately 0.1ng/ml as a capture antibody.

Applications for MED21 Antibody (6B6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MED21

MED21 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Alternate Names

hSrb7, mediator complex subunit 21SRB7 (suppressor of RNA polymerase B, yeast) homolog, RNA polymerase II complex component SRB7, RNA polymerase II holoenzyme component SRB7, RNAPII complex component SRB7, SRB7 suppressor of RNA polymerase B homolog, SRB7SRB7 suppressor of RNA polymerase B homolog (yeast), SURB7mediator of RNA polymerase II transcription subunit 21

Entrez Gene IDs

9412 (Human)

Gene Symbol

MED21

OMIM

603800 (Human)

UniProt

AAH08380 (Human)

Additional MED21 Products

Product Documents for MED21 Antibody (6B6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MED21 Antibody (6B6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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