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MED21 Antibody (PCRP-MED21-4B5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14069

Novus Biologicals, part of Bio-Techne
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NBP3-14069

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-MED21-4B5

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length human MED21 protein (Uniprot: Q13503)

Localization

Nucleus, Nucleoplasm, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13814).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for MED21 Antibody (PCRP-MED21-4B5) - Azide and BSA Free

Protein Array: MED21 Antibody (PCRP-MED21-4B5) - Azide and BSA Free [NBP3-14069]

Protein Array: MED21 Antibody (PCRP-MED21-4B5) - Azide and BSA Free [NBP3-14069]

Protein Array: MED21 Antibody (PCRP-MED21-4B5) - Azide and BSA Free [NBP3-14069] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MED21 Antibody (PCRP-MED21-4B5).

Applications for MED21 Antibody (PCRP-MED21-4B5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: MED21

MED21 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Alternate Names

hSrb7, mediator complex subunit 21SRB7 (suppressor of RNA polymerase B, yeast) homolog, RNA polymerase II complex component SRB7, RNA polymerase II holoenzyme component SRB7, RNAPII complex component SRB7, SRB7 suppressor of RNA polymerase B homolog, SRB7SRB7 suppressor of RNA polymerase B homolog (yeast), SURB7mediator of RNA polymerase II transcription subunit 21

Gene Symbol

MED21

Additional MED21 Products

Product Documents for MED21 Antibody (PCRP-MED21-4B5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MED21 Antibody (PCRP-MED21-4B5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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