Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot (Negative)

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MED23 Antibodies »

Product Specifications

Immunogen

A synthetic peptide that maps to a region between residues 1 and 50 of human Cofactor Required for Sp1 transcriptional activation, subunit 3 using the numbering given in Swiss-Prot entry Q9ULK4 (GeneID 9439).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25892301).

Specificity

NB200-337 is specific for human CRSP 3 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MED23 Antibody

Immunoprecipitation: MED23 Antibody [NB200-337]

Immunoprecipitation: MED23 Antibody [NB200-337]

Immunoprecipitation: MED23 Antibody [NB200-337] - Nuclear extract (5 mg) from HeLa cells. Antibody used at 4 ug/5 mg extract.

Applications for MED23 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunoprecipitation

1-4 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Western Blot: Not Recommended, use NB 200-339 for WB. *In some cases, the antibody may be diluted further than indicated. Use in chromatin immunoprecipitation reported in scientific literature (PMID 25892301)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MED23

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Alternate Names

130 kDa transcriptional co-activator, 133 kDa transcriptional co-activator, CRSP130, DRIP130DKFZp434H0117, mediator complex subunit 23CRSP133, Protein sur-2 homolog, subunit 3, 130kDa, Transcriptional coactivator CRSP130, Vitamin D3 receptor-interacting protein complex 130 kDa component

Entrez Gene IDs

9439 (Human)

Gene Symbol

MED23

Additional MED23 Products

Product Documents for MED23 Antibody

Product Datasheets

Certificate of Analysis

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Download SDS

Product Specific Notices for MED23 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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