MED4 Antibody (2B10)
Novus Biologicals, part of Bio-Techne | Catalog # H00029079-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2B10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MED4 (AAH05189, 1 a.a. ~ 270 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAASSSGEKEKERLGGGLGVAGGNSTRERLLSALEDLEVLSRELIEMLAISRNQKLLQAGEENQVLELLIHRDGEFQELMKLALNQGKIHHEMQVLEKEVEKRDGDIQQLQKQLKEAEQILATAVYQAKEKLKSIEKARKGAISSEEIIKYAHRISASNAVCAPLTWVPGDPRRPYPTDLEMRSGLLGQMNNPSTNGVNGHLPGDALAAGRLPDVLAPQYPWQSNDMSMNMLPPNHSSDFLLEPPGHNKEDEDDVEIMSTDSSSSSSESD
Specificity
MED4 - mediator of RNA polymerase II transcription, subunit 4 homolog (yeast) (2B10)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for MED4 Antibody (2B10)
![Western Blot: MED4 Antibody (2B10) [H00029079-M01]](https://resources.bio-techne.com/images/products/MED4-Antibody-2B10-Western-Blot-H00029079-M01-img0004.jpg "Western Blot: MED4 Antibody (2B10) [H00029079-M01]")
Western Blot: MED4 Antibody (2B10) [H00029079-M01]
Western Blot: MED4 Antibody (2B10) [H00029079-M01] - Analysis of MED4 expression in transfected 293T cell line by MED4 monoclonal antibody (M01), clone 2B10.Lane 1: MED4 transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: MED4 Antibody (2B10) [H00029079-M01]](https://resources.bio-techne.com/images/products/MED4-Antibody-2B10-Immunocytochemistry-Immunofluorescence-H00029079-M01-img0002.jpg "Immunocytochemistry/ Immunofluorescence: MED4 Antibody (2B10) [H00029079-M01]")
Immunocytochemistry/ Immunofluorescence: MED4 Antibody (2B10) [H00029079-M01]
Immunocytochemistry/Immunofluorescence: MED4 Antibody (2B10) [H00029079-M01] - Analysis of monoclonal antibody to MED4 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: MED4 Antibody (2B10) [H00029079-M01] - Detection limit for recombinant GST tagged MED4 is 1 ng/ml as a capture antibody.
Applications for MED4 Antibody (2B10)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MED4
Long Name
Mediator Complex Subunit 4
Alternate Names
ARC36, DRIP36, HSPC126, TRAP36, VDRIP
Entrez Gene IDs
29079 (Human)
Gene Symbol
MED4
UniProt
Additional MED4 Products
Product Documents for MED4 Antibody (2B10)
Product Specific Notices for MED4 Antibody (2B10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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