MED4 Antibody (8T5C9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15287

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15287-100ul
NBP3-15287-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8T5C9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MED4 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 150-250 of human MED4 (Q9NPJ6). KYAHRISASNAVCAPLTWVPGDPRRPYPTDLEMRSGLLGQMNNPSTNGVNGHLPGDALAAGRLPDVLAPQYPWQSNDMSMNMLPPNHSSDFLLEPPGHNKE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MED4 Antibody (8T5C9)

Western Blot: MED4 Antibody (8T5C9) [NBP3-15287]

Western Blot: MED4 Antibody (8T5C9) [NBP3-15287]

Western Blot: MED4 Antibody (8T5C9) [NBP3-15287] - Western blot analysis of extracts of various cell lines, using MED4 antibody (NBP3-15287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287]

Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287]

Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287] - Immunohistochemistry of paraffin-embedded rat liver using MED4 Rabbit mAb (NBP3-15287) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287]

Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287]

Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287] - Immunohistochemistry of paraffin-embedded human colon carcinoma using MED4 Rabbit mAb (NBP3-15287) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for MED4 Antibody (8T5C9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MED4

MED4 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Long Name

Mediator Complex Subunit 4

Alternate Names

ARC36, DRIP36, HSPC126, TRAP36, VDRIP

Gene Symbol

MED4

Additional MED4 Products

Product Documents for MED4 Antibody (8T5C9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MED4 Antibody (8T5C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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