MED4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84977

Novus Biologicals, part of Bio-Techne
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NBP1-84977
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Primate

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MED4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SGEKEKERLGGGLGVAGGNSTRERLLSALEDLEVLSRELIEMLAISRNQKLLQAGEENQVLELLIHRDGEFQELMKLALNQGKIHHEMQVLEKEVEKRDSDIQQLQKQLKEAEQI

Reactivity Notes

Primate reactivity reported in the scientific literature (PMID: 26067369).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MED4 Antibody

Western Blot: MED4 Antibody [NBP1-84977]

Western Blot: MED4 Antibody [NBP1-84977]

Western Blot: MED4 Antibody [NBP1-84977] - Components from all modules of Mediator are found in TAP-wtICP4 samples. Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses at 6 hpi. Results of the Western blot analysis for components of the head (Med6), middle (Med4 and Med1), tail (Med23), kinase (cdk8, Med12, and Med13), and the Med26 protein are shown. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0078242), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: MED4 Antibody [NBP1-84977]

Immunocytochemistry/ Immunofluorescence: MED4 Antibody [NBP1-84977]

Immunocytochemistry/Immunofluorescence: MED4 Antibody [NBP1-84977] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977]

Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977]

Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977] - Staining of human Placenta shows strong nuclear positivity in trophoblastic cells.
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Applications for MED4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:100-1:500
Application Notes
WB reported in scientific literature (PMID: 24147125). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MED4

MED4 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Long Name

Mediator Complex Subunit 4

Alternate Names

ARC36, DRIP36, HSPC126, TRAP36, VDRIP

Gene Symbol

MED4

Additional MED4 Products

Product Documents for MED4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MED4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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