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MED8 Antibody (1D3)

Novus Biologicals, part of Bio-Techne | Catalog # H00112950-M01

Novus Biologicals, part of Bio-Techne
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H00112950-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MED8 (NP_443109, 1 a.a. ~ 59 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQIQSLNKMCS

Reactivity Notes

Human. Other species not tested.

Specificity

MED8 - mediator of RNA polymerase II transcription, subunit 8 homolog (yeast)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for MED8 Antibody (1D3)

Immunocytochemistry/ Immunofluorescence: MED8 Antibody (1D3) [H00112950-M01]

Immunocytochemistry/ Immunofluorescence: MED8 Antibody (1D3) [H00112950-M01]

Immunocytochemistry/Immunofluorescence: MED8 Antibody (1D3) [H00112950-M01] - Analysis of monoclonal antibody to MED8 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: MED8 Antibody (1D3) [H00112950-M01] - Detection limit for recombinant GST tagged MED8 is approximately 0.1ng/ml as a capture antibody.

Applications for MED8 Antibody (1D3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MED8

This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.

Alternate Names

ARC32subunit 8 homolog, mediator complex subunit 8mediator of RNA polymerase II transcription, subunit 8 homolog (S. cerevisiae)

Entrez Gene IDs

112950 (Human)

Gene Symbol

MED8

OMIM

607956 (Human)

Additional MED8 Products

Product Documents for MED8 Antibody (1D3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MED8 Antibody (1D3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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