MEF2D Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80567
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 475 and 521 of human Myocyte Enhancer Factor 2D using the numbering given in entry NP_005911.1 (GeneID 4209).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MEF2D Antibody
![Western Blot: MEF2D Antibody [NBP1-80567]](https://resources.bio-techne.com/images/products/MEF2D-Antibody-Western-Blot-NBP1-80567-img0001.jpg "Western Blot: MEF2D Antibody [NBP1-80567]")
Western Blot: MEF2D Antibody [NBP1-80567]
Western Blot: MEF2D Antibody [NBP1-80567] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-MEF2D antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. MEF2D was also immunoprecipitated by rabbit anti-MEF2D antibody NBP1-80566, which recognizes an upstream epitope.![Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567]](https://resources.bio-techne.com/images/products/MEF2D-Antibody-Immunohistochemistry-NBP1-80567-img0006.jpg "Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567]")
Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567]
Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- MEF2D used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).![Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567]](https://resources.bio-techne.com/images/products/MEF2D-Antibody-Immunohistochemistry-Paraffin-NBP1-80567-img0005.jpg "Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567]")
Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567]
Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567] - FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-MEF2D, left image and 1, right image) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).Applications for MEF2D Antibody
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100- 1:500
Immunoprecipitation
2-10 ug/mg
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MEF2D
Alternate Names
DKFZp686I1536, MADS box transcription enhancer factor 2, polypeptide D (myocyte enhancerfactor 2D), MEF2D/DAZAP1 fusion, myocyte enhancer factor 2D, myocyte enhancer factor 2D/deleted in azoospermia associated protein 1 fusionprotein, myocyte-specific enhancer factor 2D
Gene Symbol
MEF2D
UniProt
Additional MEF2D Products
Product Documents for MEF2D Antibody
Product Specific Notices for MEF2D Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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