MEKK4 Antibody (4F10)

Novus Biologicals, part of Bio-Techne | Catalog # H00004216-M08

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4F10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MEKK4 Antibodies »

Product Specifications

Immunogen

MAP3K4 (NP_005913, 1201 a.a. ~ 1300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AASRPSPSGGDSVLPKSISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRGDSSGSTRRSWELRTLISQSKDTASKLGPIEAIQKS

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

MAP3K4 - mitogen-activated protein kinase kinase kinase 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MEKK4 Antibody (4F10)

Western Blot: MEKK4 Antibody (4F10) [H00004216-M08]

Western Blot: MEKK4 Antibody (4F10) [H00004216-M08]

Western Blot: MEKK4 Antibody (4F10) [H00004216-M08] - Analysis of MAP3K4 expression in NIH/3T3 (Cat # L018V1).
Immunohistochemistry-Paraffin: MEKK4 Antibody (4F10) [H00004216-M08]

Immunohistochemistry-Paraffin: MEKK4 Antibody (4F10) [H00004216-M08]

Immunohistochemistry-Paraffin: MEKK4 Antibody (4F10) [H00004216-M08] - Analysis of monoclonal antibody to MAP3K4 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 1 ug/ml
Sandwich ELISA: MEKK4 Antibody (4F10) [H00004216-M08] - Detection limit for recombinant GST tagged MAP3K4 is approximately 0.1ng/ml as a capture antibody.

Applications for MEKK4 Antibody (4F10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MEKK4

The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described.

Alternate Names

EC 2.7.11.25, FLJ42439, MAPKKK4yeast, homolog of, MEKK 4, mitogen-activated protein kinase kinase kinase 4

Entrez Gene IDs

4216 (Human)

Gene Symbol

MAP3K4

OMIM

602425 (Human)

UniProt

NP_005913 (Human)

Additional MEKK4 Products

Product Documents for MEKK4 Antibody (4F10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MEKK4 Antibody (4F10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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