Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MEKK4 Antibodies »

Product Summary for MEKK4 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 75 to 125 of human Mitogen-Activated Protein Kinase Kinase Kinase 4 using the numbering given in entry NP_005913.2 (GeneID 4216).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEKK4 Antibody

Western Blot: MEKK4 Antibody [NBP2-32187]

Western Blot: MEKK4 Antibody [NBP2-32187]

Western Blot: MEKK4 Antibody [NBP2-32187] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells. Antibodies: Affinity purified rabbit anti-MEKK4 antibody NBP2-32187 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: MEKK4 Antibody [NBP2-32187]

Immunoprecipitation: MEKK4 Antibody [NBP2-32187]

Immunoprecipitation: MEKK4 Antibody [NBP2-32187] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-MEKK4 antibody NBP2-32187 used for IP at 6 ug per reaction. MEKK4 was also immunoprecipitated by rabbit anti-MEKK4 antibody BL15818. For blotting immunoprecipitated MEKK4, NBP2-32187 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for MEKK4 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MEKK4

MEK kinase 4 (MEKK4), also known as MTK1, is a Ser/Thr protein kinase belonging to the MAP kinase kinase kinase family (1). This protein contains a protein kinase catalytic domain at the C-terminus. The N-terminal, non-kinase domain may contain a regulatory domain. MEKK4 regulates p38 and JNK, butnot ERK pathway, through the phosphorylation of the MAP2Ks MKK3 and MKK6 and MKK4 and MKK7 respectively (1-2).

Alternate Names

EC 2.7.11.25, FLJ42439, MAPKKK4yeast, homolog of, MEKK 4, mitogen-activated protein kinase kinase kinase 4

Gene Symbol

MAP3K4

UniProt

NP_005913.2 (Human)

Additional MEKK4 Products

Product Documents for MEKK4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MEKK4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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