MEKK4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35705
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEKK4 (NP_005913.2).
Sequence:
MREAAAALVPPPAFAVTPAAAMEEPPPPPPPPPPPPEPETESEPECCLAARQEGTLGDSACKSPESDLEDFSDETNTENLYGTSPPSTPRQMKRMSTKHQ
Sequence:
MREAAAALVPPPAFAVTPAAAMEEPPPPPPPPPPPPEPETESEPECCLAARQEGTLGDSACKSPESDLEDFSDETNTENLYGTSPPSTPRQMKRMSTKHQ
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
182 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MEKK4 Antibody
Immunohistochemistry: MEKK4 Antibody [NBP3-35705] -
Immunohistochemistry: MEKK4 Antibody [NBP3-35705] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MEKK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Western Blot: MEKK4 Antibody [NBP3-35705] -
Western Blot: MEKK4 Antibody [NBP3-35705] - Western blot analysis of various lysates using MEKK4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry: MEKK4 Antibody [NBP3-35705] -
Immunohistochemistry: MEKK4 Antibody [NBP3-35705] - Immunohistochemistry analysis of paraffin-embedded Human placenta using MEKK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.Applications for MEKK4 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MEKK4
Alternate Names
EC 2.7.11.25, FLJ42439, MAPKKK4yeast, homolog of, MEKK 4, mitogen-activated protein kinase kinase kinase 4
Gene Symbol
MAP3K4
Additional MEKK4 Products
Product Documents for MEKK4 Antibody
Product Specific Notices for MEKK4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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