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MEL-18 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13739

Novus Biologicals, part of Bio-Techne
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NBP2-13739
NBP2-13739-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for MEL-18 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLSIEFYEGARDRDEKKGPLENGDGDKEKTGVRFLRCP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEL-18 Antibody

Immunohistochemistry-Paraffin: MEL-18 Antibody [NBP2-13739]

Immunohistochemistry-Paraffin: MEL-18 Antibody [NBP2-13739]

Immunohistochemistry-Paraffin: MEL-18 Antibody [NBP2-13739] Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Applications for MEL-18 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEL-18

MEL-18 is a transcriptional repressor that binds specifically to the DNA sequence 5'-GACTNGACT-3'. Mel18 has tumor suppressor activity and may play a role in cell proliferation and/or neural cell development. It regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells.

Alternate Names

MEL-18, polycomb group ring finger 2, polycomb group RING finger protein 2, RING finger protein 110DNA-binding protein Mel-18, RNF110MEL18, Zinc finger protein 144, ZNF144MGC10545

Gene Symbol

PCGF2

Additional MEL-18 Products

Product Documents for MEL-18 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MEL-18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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