Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34337

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda/IgG2a Kappa/IgG1 Kappa Clone # M2-7C10 + M2-9E3 + T311 + HMB45

Concentration

0.2 mg/ml

View all Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibodies »

Product Specifications

Immunogen

Recombinant Melanoma Marker (MART-1 + Tyrosinase + gp100) protein (M2-7C10; M2-9E3); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Localization

Cytoplasmic

Specificity

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Marker

Melanoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda/IgG2a Kappa/IgG1 Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34681)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images

Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]

Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]

Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Western Blot Analysis of COLO38 cell lysate using Melanoma Marker (MART-1 + Tyrosinase + gp100) MAb (M2-7C10 + M2-9E3 + T311 + HMB45).
Immunohistochemistry: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]

Immunohistochemistry: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]

Immunohistochemistry: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Scan of human melanoma sample. Stained for Mart-1+Tyrosinase+gp100 (green) counter stained with DAPI (blue). Image from verified customer review. Image using the Janelia Fluor 549 format of this antibody.
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Formalin-fixed, paraffin-embedded human melanoma stained with Melanoma Marker MAb (M2-7C10 + M2-9E3 + T311 + HMB45).
View 2 more images

Applications

Application
Recommended Usage

Immunohistochemistry-Frozen

0.5-1ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Melanoma Marker (MART-1 + Tyrosinase + gp100)

Alternate Names

MART1, MART-1, melan-A, MLANA

Gene Symbol

MLANA

UniProt

Q16655, P14679 (Human), P40967 (Human)

Additional Melanoma Marker (MART-1 + Tyrosinase + gp100) Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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