Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34337
![Immunohistochemistry-Frozen: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]](https://resources.bio-techne.com/images/products/Melanoma-Marker-MART-1-+-Tyrosinase-+-gp100-Antibody-M2-7C10-+-M2-9E3-+-T311-+-HMB45-Immunohistochemistry-Frozen-NBP2-34337-img0002.jpg "Immunohistochemistry-Frozen: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]")
![Immunohistochemistry-Frozen: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]](https://resources.bio-techne.com/images/products/Melanoma-Marker-MART-1-+-Tyrosinase-+-gp100-Antibody-M2-7C10-+-M2-9E3-+-T311-+-HMB45-Immunohistochemistry-Frozen-NBP2-34337-img0003.jpg "Immunohistochemistry-Frozen: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]")
![Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]](https://resources.bio-techne.com/images/products/Melanoma-Marker-MART-1-+-Tyrosinase-+-gp100-Antibody-M2-7C10-+-M2-9E3-+-T311-+-HMB45-Western-Blot-NBP2-34337-img0006.jpg "Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Lambda/IgG2a Kappa/IgG1 Kappa Clone # M2-7C10 + M2-9E3 + T311 + HMB45
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant Melanoma Marker (MART-1 + Tyrosinase + gp100) protein (M2-7C10; M2-9E3); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Localization
Cytoplasmic
Specificity
This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Lambda/IgG2a Kappa/IgG1 Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34681)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images
Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]
Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Western Blot Analysis of COLO38 cell lysate using Melanoma Marker (MART-1 + Tyrosinase + gp100) MAb (M2-7C10 + M2-9E3 + T311 + HMB45).![Immunohistochemistry: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]](https://resources.bio-techne.com/images/products/Melanoma-Marker-MART-1-+-Tyrosinase-+-gp100-Antibody-M2-7C10-+-M2-9E3-+-T311-+-HMB45-Immunohistochemistry-NBP2-34337-img0005.jpg "Immunohistochemistry: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]")
Immunohistochemistry: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]
Immunohistochemistry: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Scan of human melanoma sample. Stained for Mart-1+Tyrosinase+gp100 (green) counter stained with DAPI (blue). Image from verified customer review. Image using the Janelia Fluor 549 format of this antibody.![Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]](https://resources.bio-techne.com/images/products/Melanoma-Marker-MART-1-+-Tyrosinase-+-gp100-Antibody-M2-7C10-+-M2-9E3-+-T311-+-HMB45-Immunohistochemistry-Paraffin-NBP2-34337-img0001.jpg "Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]")
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337]
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [NBP2-34337] - Formalin-fixed, paraffin-embedded human melanoma stained with Melanoma Marker MAb (M2-7C10 + M2-9E3 + T311 + HMB45).Applications
Application
Recommended Usage
Immunohistochemistry-Frozen
0.5-1ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Melanoma Marker (MART-1 + Tyrosinase + gp100)
Alternate Names
MART1, MART-1, melan-A, MLANA
Gene Symbol
MLANA
UniProt
Additional Melanoma Marker (MART-1 + Tyrosinase + gp100) Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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