Melanopsin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74460

Novus Biologicals, part of Bio-Techne
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NB100-74460-0.025mg
NB100-74460
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Knockout Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Knockout Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Melanopsin Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues E(455) Q K S K T P K T K R H L P S L D R R M(474) of rat melanopsin.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 20971788). Mouse (PMID: 22886938).

Localization

Membrane; Multi-pass membrane protein. Note: Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Melanopsin Antibody

Immunocytochemistry/ Immunofluorescence: Melanopsin Antibody [NB100-74460]

Immunocytochemistry/ Immunofluorescence: Melanopsin Antibody [NB100-74460]

Immunocytochemistry/Immunofluorescence: Melanopsin Antibody [NB100-74460] - Staining in rat retinal ganglion cells.

Applications for Melanopsin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/mL

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:1000

Western Blot

0.1 - 0.2 ug/mL
Application Notes
Successful WB, IF, and ICC usages. WB: Detects approx. 53 and 85 kDa proteins representing overexpressed unglycosylated and glycosylated melanopsin from HEK 293 cells (does not work well on endogenously expressed protein). ICC/IF: NB100-74460 detects melanopsin from rat retinal ganglion cells (RGCs). Knockout data (PMID: 31924821).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 1 mg/mL BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Melanopsin

Melanopsin is an opsin-like protein found in the retinal ganglion cells of mammals. Melanopsin is believed to be part of a secondary optical system that parallels that of the classic rod and cone system. This second system reports directly to the suprachiasmatic nucleus and is responsible for regulation of circadian rhythms. Melanopsin is believed to be the primary photopigment responsible for the regulation of these circadian rhythms, and Melanopsin knockout mice have been generated which demonstrate decreased capacity to entrain to light and dark cycles.

Long Name

Melanopsin

Alternate Names

OPN4, Opsin-4

Entrez Gene IDs

30044 (Mouse); 192223 (Rat)

Gene Symbol

OPN4

Additional Melanopsin Products

Product Documents for Melanopsin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Melanopsin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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