MELK Antibody (2G2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37481

Novus Biologicals, part of Bio-Techne
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NBP2-37481
NBP2-37481-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 2G2

Format

BSA Free

Concentration

1 mg/ml

View all MELK Antibodies »

Product Summary for MELK Antibody (2G2) - BSA Free

Immunogen

Synthesized peptide of human MELK(AA: 637-651:C-VYKRLVEDILSSCKV).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

74.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MELK Antibody (2G2) - BSA Free

Immunocytochemistry/ Immunofluorescence: MELK Antibody (2G2) - BSA Free [NBP2-37481]

Immunocytochemistry/ Immunofluorescence: MELK Antibody (2G2) - BSA Free [NBP2-37481]

Immunocytochemistry/Immunofluorescence: MELK Antibody (2G2) [NBP2-37481] - Immunofluorescence analysis of HepG2 cells using MELK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: MELK Antibody (2G2) - BSA Free [NBP2-37481]

Immunohistochemistry: MELK Antibody (2G2) - BSA Free [NBP2-37481]

Immunohistochemistry: MELK Antibody (2G2) [NBP2-37481] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MELK mouse mAb with DAB staining.
Flow Cytometry: MELK Antibody (2G2) - BSA Free [NBP2-37481]

Flow Cytometry: MELK Antibody (2G2) - BSA Free [NBP2-37481]

Flow Cytometry: MELK Antibody (2G2) [NBP2-37481] - Flow cytometric analysis of MCF-7 cells using MELK mouse mAb (green) and negative control (red).
View 2 more images

Applications for MELK Antibody (2G2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MELK

MELK is a member of AMPK family, which has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press]. Further the activity of this antibody was confirmed against recombinant, GST fused MELK protein by Western blotting.

Long Name

Maternal Embryonic Leucine Zipper Kinase

Alternate Names

PK38

Gene Symbol

MELK

Additional MELK Products

Product Documents for MELK Antibody (2G2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MELK Antibody (2G2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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