MELK Antibody (PSH03-55)

Western Blot: MELK Antibody (PSH03-55) [NBP3-32587]

Western Blot: MELK Antibody (PSH03-55) [NBP3-32587] - Western blot analysis of MELK on different lysates with Rabbit anti-MELK antibody (NBP3-32587) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C2C12 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 75 kDa
Observed band size: 72 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32587) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: MELK Antibody (PSH03-55) [NBP3-32587]

Immunohistochemistry: MELK Antibody (PSH03-55) [NBP3-32587] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MELK antibody (NBP3-32587) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32587) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.