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MELK Antibody (S06-4D6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15059

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-15059-100ul
NBP3-15059-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S06-4D6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for MELK Antibody (S06-4D6)

Immunogen

A synthetic peptide of human MELK (Uniprot#: Q14680)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MELK Antibody (S06-4D6)

Western Blot: MELK Antibody (S06-4D6) [NBP3-15059]

Western Blot: MELK Antibody (S06-4D6) [NBP3-15059]

Western Blot: MELK Antibody (S06-4D6) [NBP3-15059] - Western blot detection of MELK in U2OS, C6 cell lysates using MELK Rabbit mAb (1:1000 diluted). Predicted band size: 75kDa. Observed band size: 75kDa.

Applications for MELK Antibody (S06-4D6)

Application
Recommended Usage

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MELK

MELK is a member of AMPK family, which has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press]. Further the activity of this antibody was confirmed against recombinant, GST fused MELK protein by Western blotting.

Long Name

Maternal Embryonic Leucine Zipper Kinase

Alternate Names

PK38

Gene Symbol

MELK

Additional MELK Products

Product Documents for MELK Antibody (S06-4D6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MELK Antibody (S06-4D6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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