Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Menin Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Menin Antibody

Western Blot: Menin Antibody [NB100-215]

Western Blot: Menin Antibody [NB100-215]

Western Blot: Menin Antibody [NB100-215] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Menin antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: Menin Antibody [NB100-215]

Immunohistochemistry-Paraffin: Menin Antibody [NB100-215]

Immunohistochemistry-Paraffin: Menin Antibody [NB100-215] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Menin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Western Blot: Menin Antibody [NB100-215]

Western Blot: Menin Antibody [NB100-215]

Western Blot: Menin Antibody [NB100-215] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Menin antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
View 1 more images

Applications for Menin Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-10 ug/mg of lysate

Simple Western

1:100

Western Blot

1:10000 - 1:25000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. Simple Western reported by an internal validation: tested in MCF-7, T-47D cell lines; antibody dilution of 1:100; separated by size
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Menin

Menin is the gene product of the putative tumor suppressor MEN1 associated with the syndrome termed multiple endocrine neoplasia type 1 that may function in the regulation of DNA synthesis or the response to DNA damage. Menin has been shown to interact with JunD, a member of the AP-1 heterodimer and inhibit transcription. Menin has also been shown to interact with Pem, FANCD2, RPA, NMMHCII-A, GFAP, vimentin, Hsp70, NfkappaB proteins, Smads, mSin3A and is part of the MLL2 and MLL1 Set1-like histone H3 K4 methyltransferase complex where it interacts directly with ASH2, Rbbp5, and WDR5. As part of the MLL complexes, menin may mediate its tumor suppressor activity via the regulation of histone methylation of HOX and CDK inhibitor genes. Alternate names for Menin are MEAI, multiple endocrine adenomatosis 1, suppressor Candidate Gene 2, and SCG2.

Long Name

Multiple Endocrine Neoplasia I

Alternate Names

MEAI, MEN1

Entrez Gene IDs

4221 (Human); 17283 (Mouse)

Gene Symbol

MEN1

UniProt

O00255 (Human)

Additional Menin Products

Product Documents for Menin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Menin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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