Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

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Product Summary for Menin Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Menin Antibody

Western Blot: Menin Antibody [NB100-287]

Western Blot: Menin Antibody [NB100-287]

Western Blot: Menin Antibody [NB100-287] - Using the Biotin direct conjugate Samples: Whole cell lysate from HEK 293T (50, 15, and 5 ug). Antibody: Affinity purified, biotin-conjugated, goat anti-Menin antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.
Western Blot: Menin Antibody [NB100-287]

Western Blot: Menin Antibody [NB100-287]

Western Blot: Menin Antibody [NB100-287] - Samples: Whole cell lysate (40 ug for WB; one 10 cm plate 293T cells for IP) from 293T cells. Affinity purified goat anti-Menin used as indicated for IP. Menin was detected by Western Blot using affinity purified rabbit anti-Menin NB100-215 at 0.1 ug/ml for WB. Detection: Chemiluminescence exposed for 1 minute.
Western Blot: Menin Antibody [NB100-287]

Western Blot: Menin Antibody [NB100-287]

Western Blot: Menin Antibody [NB100-287] - Analysis using the Biotin conjugate of NB100-287. Detection of HEK 293T (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.
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Applications for Menin Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Menin

Menin is the gene product of the putative tumor suppressor MEN1 associated with the syndrome termed multiple endocrine neoplasia type 1 that may function in the regulation of DNA synthesis or the response to DNA damage. Menin has been shown to interact with JunD, a member of the AP-1 heterodimer and inhibit transcription. Menin has also been shown to interact with Pem, FANCD2, RPA, NMMHCII-A, GFAP, vimentin, Hsp70, NfkappaB proteins, Smads, mSin3A and is part of the MLL2 and MLL1 Set1-like histone H3 K4 methyltransferase complex where it interacts directly with ASH2, Rbbp5, and WDR5. As part of the MLL complexes, menin may mediate its tumor suppressor activity via the regulation of histone methylation of HOX and CDK inhibitor genes.

Long Name

Multiple Endocrine Neoplasia I

Alternate Names

MEAI, MEN1

Entrez Gene IDs

4221 (Human); 17283 (Mouse); 29417 (Rat)

Gene Symbol

MEN1

UniProt

NP_000235.2 (Human)

Additional Menin Products

Product Documents for Menin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Menin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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