MERS-CoV Spike Protein Antibody (723)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90336
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
MERS-CoV
Applications
Block/Neutralize
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 723
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant MERS-CoV (Accession#: AFS88936.1; Met1-Trp1297).
Specificity
Has cross-reactivity in ELISA with: MERS-CoV Spike Protein (aa 1-1297)MERS-CoV Spike Protein S1 (aa 1-725)MERS-CoV Spike Protein RBD (aa 367-606)No cross-reactivity in ELISA with: MERS-CoV Spike Protein S2 (aa 726-1296)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for MERS-CoV Spike Protein Antibody (723)
Block/Neutralize: MERS-CoV Spike Protein Antibody (723) [NBP2-90336] - MERS-CoV Spike Protein antibody (723) measured by its ability to inhibit infection of Caco-2 cells induced by MERS-CoV pseudovirus. The ED50 for this effect is 2.5-11 ng/mL.
Applications for MERS-CoV Spike Protein Antibody (723)
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Microneutralization: Neutralization of MERS-CoV pseudovirus by NBP2-90336. Positive Control:293T/DPP4 cells were infected with MERS-CoV pseudovirus; Negative Control:293T/DPP4 cells without MERS-CoV pseudovirus; NBP2-90336: MERS-CoV pseudovirus infection in 293T/DPP4 cells was inhibited by NBP2-90336. Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MERS-CoV Spike Protein
Alternate Names
MERS S glycoprotein, MERS S1, MERS S2, MERS Spike glycoprotein, MERS Spike Protein, MERS-CoV S glycoprotein, Middle East respiratory syndrome-related coronavirus Spike glycoprotein, Spike glycoprotein, spike protein, Spike RBD, surface glycoprotein
Gene Symbol
S
UniProt
Additional MERS-CoV Spike Protein Products
Product Documents for MERS-CoV Spike Protein Antibody (723)
Product Specific Notices for MERS-CoV Spike Protein Antibody (723)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...