MEST Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04728
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 146-335 of human MEST (NP_002393.2). HDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPLLLQKLLKDGGVLSPILTRLMNFFVFSRGLTPVFGPYTRPSESELWDMWAGIRNNDGNLVIDSLLQYINQRKKFRRRWVGALASVTIPIHFIYGPLDPVNPYPEFLELYRKTLPRSTVSILDDHISHYPQLEDPMGFLNAYMGFINSF
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MEST Antibody - BSA Free
Western Blot: MEST Antibody - BSA Free [NBP3-04728] -
Western Blot: MEST Antibody - BSA Free [NBP3-04728] - Western blot analysis of Mouse brain, using MEST antibody (A6931) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
![Immunocytochemistry/ Immunofluorescence: MEST Antibody - BSA Free [NBP3-04728]](https://resources.bio-techne.com/images/products/MEST-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04728-img0001.jpg "Immunocytochemistry/ Immunofluorescence: MEST Antibody - BSA Free [NBP3-04728]")
Immunocytochemistry/ Immunofluorescence: MEST Antibody - BSA Free [NBP3-04728]
Immunocytochemistry/Immunofluorescence: MEST Antibody [NBP3-04728] - Analysis of U2OS cells using MEST antibody . Blue: DAPI for nuclear staining.Applications for MEST Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Western Blot
1:1000 - 1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MEST
Alternate Names
DKFZp686L18234, EC 3.-, mesoderm specific transcript (mouse) homolog, mesoderm specific transcript homolog (mouse), mesoderm-specific transcript homolog protein, MGC111102, MGC8703, paternally expressed gene 1, PEG1Paternally-expressed gene 1 protein
Gene Symbol
MEST
Additional MEST Products
Product Documents for MEST Antibody - BSA Free
Product Specific Notices for MEST Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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