Metadherin Antibody (CL0397)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52929

Novus Biologicals, part of Bio-Techne
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NBP2-52929
NBP2-52929-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0397

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Metadherin Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RKTEPSAWSQDTGDANTNGKDWGRSWSDRSIFSGIGSTAEPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPNSDWNAPAEEWGNWVDEERASLLKSQEPIPDDQKVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSTAQD

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Metadherin Antibody (CL0397)

Western Blot: Metadherin Antibody (CL0397) [NBP2-52929]

Western Blot: Metadherin Antibody (CL0397) [NBP2-52929]

Western Blot: LYRIC Antibody (CL0397) [NBP2-52929] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: Metadherin Antibody (CL0397) [NBP2-52929]

Immunocytochemistry/ Immunofluorescence: Metadherin Antibody (CL0397) [NBP2-52929]

Immunocytochemistry/Immunofluorescence: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of U-251 cells using the Anti-MTDH monoclonal antibody, showing specific staining in the endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Metadherin Antibody (CL0397) [NBP2-52929]

Immunohistochemistry-Paraffin: Metadherin Antibody (CL0397) [NBP2-52929]

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human testis shows strong cytoplasmic positivity in seminiferous tubules.
View 6 more images

Applications for Metadherin Antibody (CL0397)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Metadherin

LYRIC is a type II membrane protein, the precise role of which has yet to be defined. It is expressed in breast and prostate carcinoma and is a potential mediator of lung metastasis. It has also been found in mouse Purkinje neurons and cerebellum. In epithelial cells it is recruited to tight junctions (TJ) during the maturation of the TJ complexes. LYRIC co-localizes with beta-catenin in normal and tumour tissues.

Alternate Names

3D3, AEG1, LYRIC, MTDH

Gene Symbol

MTDH

Additional Metadherin Products

Product Documents for Metadherin Antibody (CL0397)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Metadherin Antibody (CL0397)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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