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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

Product Specifications

Immunogen

Recognizes region between residues Between Between 336 and 386..

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Methionine Aminopeptidase 1/METAP1 Antibody

Methionine Aminopeptidase 1/METAP1 Antibody

Immunoprecipitation-Methionine Aminopeptidase 1/METAP1 Antibody-NBP3-18418-

Immunoprecipitation-Methionine Aminopeptidase 1/METAP1 Antibody-NBP3-18418-Detection of human METAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-METAP1 antibody used for IP at 2 ug per reaction. METAP1 was also immunoprecipitated by rabbit anti-METAP1 antibody. For blotting immunoprecipitated METAP1, was used at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Methionine Aminopeptidase 1/METAP1 Antibody

Western Blot-Methionine Aminopeptidase 1/METAP1 Antibody-NBP3-18418-

Western Blot-Methionine Aminopeptidase 1/METAP1 Antibody-NBP3-18418-Detection of human METAP1 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, HeLa, HEK293T, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METAP1 antibody used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Methionine Aminopeptidase 1/METAP1 Antibody

Western Blot-Methionine Aminopeptidase 1/METAP1 Antibody-NBP3-18418-

Western Blot-Methionine Aminopeptidase 1/METAP1 Antibody-NBP3-18418-Detection of mouse METAP1 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METAP1 antibody used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for Methionine Aminopeptidase 1/METAP1 Antibody

Application
Recommended Usage

Immunoprecipitation

20 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Methionine Aminopeptidase 1/METAP1

Removes the amino-terminal methionine from nascent proteins. Required for normal progression through the cell cycle

Alternate Names

MAP1, MetAP1

Gene Symbol

METAP1

Additional Methionine Aminopeptidase 1/METAP1 Products

Product Documents for Methionine Aminopeptidase 1/METAP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Methionine Aminopeptidase 1/METAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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