Methionine Aminopeptidase 1D/MAP1D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92098

Novus Biologicals, part of Bio-Techne
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NBP1-92098
NBP1-92098-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Methionine Aminopeptidase 1D/MAP1D Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KPDYVTTGIVPDWGDSIEVKNEDQIQGLHQACQLARHVLLLAGKSLKVDMTTEEIDALVHREIISHNAYPSPLGYGG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Methionine Aminopeptidase 1D/MAP1D Antibody

Western Blot: Methionine Aminopeptidase 1D/MAP1D Antibody [NBP1-92098]

Western Blot: Methionine Aminopeptidase 1D/MAP1D Antibody [NBP1-92098]

Western Blot: Methionine Aminopeptidase 1D/MAP1D Antibody [NBP1-92098] - Analysis in control (vector only transfected HEK293T lysate) and METAP1D over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: Methionine Aminopeptidase 1D/MAP1D Antibody [NBP1-92098]

Immunocytochemistry/ Immunofluorescence: Methionine Aminopeptidase 1D/MAP1D Antibody [NBP1-92098]

Immunocytochemistry/Immunofluorescence: Methionine Aminopeptidase 1D/MAP1D Antibody [NBP1-92098] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunohistochemistry-Paraffin: Methionine Aminopeptidase 1D/MAP1D Antibody [NBP1-92098]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 1D/MAP1D Antibody [NBP1-92098]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 1D/MAP1D Antibody [NBP1-92098] - Staining of human stomach shows strong cytoplasmic positivity in parietal cells.
View 1 more images

Applications for Methionine Aminopeptidase 1D/MAP1D Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Methionine Aminopeptidase 1D/MAP1D

The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).[supplied by OMIM]

Alternate Names

MAP1D, MetAP1D

Gene Symbol

METAP1D

Additional Methionine Aminopeptidase 1D/MAP1D Products

Product Documents for Methionine Aminopeptidase 1D/MAP1D Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Methionine Aminopeptidase 1D/MAP1D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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