Methionine Aminopeptidase 2/METAP2 Antibody (010)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90623
Recombinant Monoclonal Antibody
![Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623]](https://resources.bio-techne.com/images/products/Methionine-Aminopeptidase-2-METAP2-Antibody-010-Western-Blot-NBP2-90623-img0003.jpg "Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 010
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Methionine Aminopeptidase 2/METAP2 (Uniprot#: O08663; Ala2-Tyr478).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Methionine Aminopeptidase 2/METAP2 Antibody (010)
Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623]
Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623] - Anti-Methionine Aminopeptidase 2/METAP2 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 52 kDa. Observed band size: 65 kDa![Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623]](https://resources.bio-techne.com/images/products/Methionine-Aminopeptidase-2-METAP2-Antibody-010-Immunoprecipitation-NBP2-90623-img0004.jpg "Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623]")
Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623]
Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623] - Mouse Methionine Aminopeptidase 2/METAP2 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-Mouse Methionine Aminopeptidase 2/METAP2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Methionine Aminopeptidase 2/METAP2 rabbit monoclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 52 kDa. Observed band size: 72 kDaApplications for Methionine Aminopeptidase 2/METAP2 Antibody (010)
Application
Recommended Usage
Immunoprecipitation
0.5-2 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Methionine Aminopeptidase 2/METAP2
Alternate Names
Amp2, METAP2, MNPEP, p67eIF2
Gene Symbol
METAP2
Additional Methionine Aminopeptidase 2/METAP2 Products
Product Documents for Methionine Aminopeptidase 2/METAP2 Antibody (010)
Product Specific Notices for Methionine Aminopeptidase 2/METAP2 Antibody (010)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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