Methionine Aminopeptidase 2/METAP2 Antibody (010)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90623

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 010

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Methionine Aminopeptidase 2/METAP2 Antibodies »

Product Summary for Methionine Aminopeptidase 2/METAP2 Antibody (010)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Methionine Aminopeptidase 2/METAP2 (Uniprot#: O08663; Ala2-Tyr478).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Methionine Aminopeptidase 2/METAP2 Antibody (010)

Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623]

Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623]

Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623] - Anti-Methionine Aminopeptidase 2/METAP2 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 52 kDa. Observed band size: 65 kDa
Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623]

Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623]

Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody (010) [NBP2-90623] - Mouse Methionine Aminopeptidase 2/METAP2 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-Mouse Methionine Aminopeptidase 2/METAP2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Methionine Aminopeptidase 2/METAP2 rabbit monoclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 52 kDa. Observed band size: 72 kDa

Applications for Methionine Aminopeptidase 2/METAP2 Antibody (010)

Application
Recommended Usage

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Methionine Aminopeptidase 2/METAP2

METAP2 is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. [provided by RefSeq]

Alternate Names

Amp2, METAP2, MNPEP, p67eIF2

Gene Symbol

METAP2

Additional Methionine Aminopeptidase 2/METAP2 Products

Product Documents for Methionine Aminopeptidase 2/METAP2 Antibody (010)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Methionine Aminopeptidase 2/METAP2 Antibody (010)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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