Methionine Aminopeptidase 2/METAP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99115

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Methionine Aminopeptidase 2/METAP2 Antibodies »

Product Summary for Methionine Aminopeptidase 2/METAP2 Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human Methionine Aminopeptidase 2/METAP2 (Accession#: NP_006829.1; Met1-Tyr478)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Methionine Aminopeptidase 2/METAP2 Antibody

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] - Immunochemical staining of human Methionine Aminopeptidase 2/METAP2 in human small intestine with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Methionine Aminopeptidase 2/METAP2 Antibody

Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -

Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -Lane A: HepG2 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: PC12 Whole Cell LysateLysates/proteins at 30 μg per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.)
Methionine Aminopeptidase 2/METAP2 Antibody

Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -

Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -Lane A:0.5 mg HepG2 Whole Cell Lysate4 µL anti-METAP2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-METAP2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 72 kDa
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Applications for Methionine Aminopeptidase 2/METAP2 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry

1:2500-1:10000

Immunohistochemistry-Paraffin

1:2500-1:10000

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Methionine Aminopeptidase 2/METAP2

METAP2 is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. [provided by RefSeq]

Alternate Names

Amp2, METAP2, MNPEP, p67eIF2

Gene Symbol

METAP2

Additional Methionine Aminopeptidase 2/METAP2 Products

Product Documents for Methionine Aminopeptidase 2/METAP2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Methionine Aminopeptidase 2/METAP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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