Methionine Aminopeptidase 2/METAP2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99115
![Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115]](https://resources.bio-techne.com/images/products/Methionine-Aminopeptidase-2-METAP2-Antibody-Immunohistochemistry-Paraffin-NBP2-99115-img0004.jpg "Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human Methionine Aminopeptidase 2/METAP2 (Accession#: NP_006829.1; Met1-Tyr478)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Methionine Aminopeptidase 2/METAP2 Antibody
Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115]
Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] - Immunochemical staining of human Methionine Aminopeptidase 2/METAP2 in human small intestine with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -
Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -Lane A: HepG2 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: PC12 Whole Cell LysateLysates/proteins at 30 μg per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.)
Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -
Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -Lane A:0.5 mg HepG2 Whole Cell Lysate4 µL anti-METAP2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-METAP2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 72 kDaApplications for Methionine Aminopeptidase 2/METAP2 Antibody
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunohistochemistry
1:2500-1:10000
Immunohistochemistry-Paraffin
1:2500-1:10000
Immunoprecipitation
4-8 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Methionine Aminopeptidase 2/METAP2
Alternate Names
Amp2, METAP2, MNPEP, p67eIF2
Gene Symbol
METAP2
Additional Methionine Aminopeptidase 2/METAP2 Products
Product Documents for Methionine Aminopeptidase 2/METAP2 Antibody
Product Specific Notices for Methionine Aminopeptidase 2/METAP2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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