Methylglyoxal Antibody (9E7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59368

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Non-species specific

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 9E7

Concentration

1 mg/ml

View all Methylglyoxal Antibodies »

Product Specifications

Immunogen

Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Methylglyoxal Antibody (9E7)

Western Blot: Methylglyoxal Antibody (9E7) [NBP2-59368]

Western Blot: Methylglyoxal Antibody (9E7) [NBP2-59368]

Western Blot: Methylglyoxal Antibody (9E7) [NBP2-59368] - Western Blot analysis of Methylglyoxal-BSA Conjugate showing detection of 67 kDa Methylglyoxal protein using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7 (NBP2-59368). Lane 1: Molecular Weight Ladder (MW). Lane 2: Methylglyoxal-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (NBP2-59368) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Immunocytochemistry/ Immunofluorescence: Methylglyoxal Antibody (9E7) [NBP2-59368]

Immunocytochemistry/ Immunofluorescence: Methylglyoxal Antibody (9E7) [NBP2-59368]

Immunocytochemistry/Immunofluorescence: Methylglyoxal Antibody (9E7) [NBP2-59368] - Tissue: Humnan embryonic kidney cells (HEK293). Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (SMC-516) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Immunohistochemistry: Methylglyoxal Antibody (9E7) [NBP2-59368]

Immunohistochemistry: Methylglyoxal Antibody (9E7) [NBP2-59368]

Immunohistochemistry: Methylglyoxal Antibody (9E7) [NBP2-59368] - Immunohistochemistry analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7 (NBP2-59368). Tissue: Heart. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (NBP2-59368) at 1:25 for 3 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Actin (C) Methylgyloxal Antibody (D) Composite.
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Applications for Methylglyoxal Antibody (9E7)

Application
Recommended Usage

ELISA

1:1000

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Methylglyoxal

Methylglyoxal is the organic compound with the formula CH3C(O)CHO. Gaseous methylglyoxal has two carbonyl groups, an aldehyde and a ketone but in the presence of water, it exists as hydrates and oligomers. It is a reduced derivative of pyruvic acid.

Alternate Names

2-oxopropanal, Pyruvaldehyde

Additional Methylglyoxal Products

Product Documents for Methylglyoxal Antibody (9E7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Methylglyoxal Antibody (9E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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