Methylmalonyl Coenzyme A mutase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38063
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 451-750 of human Methylmalonyl Coenzyme A mutase (NP_000246.2).
Sequence:
EMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDAVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGISTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLFEVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV
Sequence:
EMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDAVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGISTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLFEVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Methylmalonyl Coenzyme A mutase Antibody
Immunocytochemistry/ Immunofluorescence: Methylmalonyl Coenzyme A mutase Antibody [NBP3-38063] -
Immunocytochemistry/ Immunofluorescence: Methylmalonyl Coenzyme A mutase Antibody [NBP3-38063] - Immunofluorescence analysis of L929 cells using Methylmalonyl Coenzyme A mutase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Methylmalonyl Coenzyme A mutase Antibody [NBP3-38063] -
Immunocytochemistry/ Immunofluorescence: Methylmalonyl Coenzyme A mutase Antibody [NBP3-38063] - Immunofluorescence analysis of U-2 OS cells using Methylmalonyl Coenzyme A mutase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western Blot: Methylmalonyl Coenzyme A mutase Antibody [NBP3-38063] -
Western Blot: Methylmalonyl Coenzyme A mutase Antibody [NBP3-38063] - Western blot analysis of various lysates using Methylmalonyl Coenzyme A mutase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Applications for Methylmalonyl Coenzyme A mutase Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:1000 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Methylmalonyl Coenzyme A mutase
Alternate Names
EC 5.4.99.2, MCMmethylmalonyl Coenzyme A mutase, methylmalonyl CoA mutase, Methylmalonyl-CoA isomerase, methylmalonyl-CoA mutase, mitochondrial
Gene Symbol
MMUT
Additional Methylmalonyl Coenzyme A mutase Products
Product Documents for Methylmalonyl Coenzyme A mutase Antibody
Product Specific Notices for Methylmalonyl Coenzyme A mutase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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