Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Methyltransferase like 3 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 530 and 580 of human methyltransferase like 3 (N6-adenosine-methyltransferase 70 kDa subunit) using the numbering given in entry NP_062826.2 (GeneID 56339).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Methyltransferase like 3 Antibody

Immunohistochemistry-Paraffin: Methyltransferase like 3 Antibody [NB100-77311]

Immunohistochemistry-Paraffin: Methyltransferase like 3 Antibody [NB100-77311]

Immunohistochemistry-Paraffin: Methyltransferase like 3 Antibody [NB100-77311] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- METTL3/MT-A70 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB
Immunoprecipitation: Methyltransferase like 3 Antibody [NB100-77311]

Immunoprecipitation: Methyltransferase like 3 Antibody [NB100-77311]

Immunoprecipitation: Methyltransferase like 3 Antibody [NB100-77311] - Detection of Human METTL3 by Western Blot of immunoprecipitants. Sample: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-METTL3/MT-A70 antibody (NB100-77311) used for IP at 3 mcg/mg lysate. METTL3/MT-A70 was also immunoprecipitated by rabbit anti-METTL3/MT-A70 antibody (NB100-77310), which recognizes an upstream epitope. For blotting immunoprecipitated METTL3/MT-A70, NB100-77310 was used at 0.1 mcg/ml Detection: Chemiluminescence with exposure times of 30 seconds (A and B).anti-METTL3/MT-A70 antibody NB100-77310, which recognizes an upstream epitope.

Applications for Methyltransferase like 3 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

2 - 5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Methyltransferase like 3

This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

Alternate Names

adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase, EC 2.1.1.62, IME4, M6A, methyltransferase like 3, Methyltransferase-like protein 3, MGC4336, mRNA m(6)A methyltransferase, MTA70, MT-A70, N6-adenosine-methyltransferase 70 kDa subunit, Spo8

Entrez Gene IDs

56339 (Human)

Gene Symbol

Q86U44

UniProt

NP_062826.2 (Human)

Additional Methyltransferase like 3 Products

Product Documents for Methyltransferase like 3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Methyltransferase like 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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