Methyltransferase like 3 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03290
![Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]](https://resources.bio-techne.com/images/products/Methyltransferase-like-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03290-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human Methyltransferase like 3 (NP_062826.2). MSDTWSSIQAHKKQLDSLRERLQRRRKQDSGHLDLRNPEAALSPTFRSDSPVPTAPTSGGPKPSTASAVPELATDPELEKKLLHHLSDLALTLPTDAVSICLAISTPDAPATQDGVESLLQKFAAQELIEVKRGLLQDDAHPTLVTYADHSKLSAM
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Methyltransferase like 3 Antibody - Azide and BSA Free
![Western Blot: Methyltransferase like 3 AntibodyAzide and BSA Free [NBP3-03290]](https://resources.bio-techne.com/images/products/Methyltransferase-like-3-Antibody-Western-Blot-NBP3-03290-img0012.jpg "Western Blot: Methyltransferase like 3 AntibodyAzide and BSA Free [NBP3-03290]")
Western Blot: Methyltransferase like 3 AntibodyAzide and BSA Free [NBP3-03290]
Western Blot: Methyltransferase like 3 Antibody [NBP3-03290] - Analysis of various lysates, using METTL3 antibody at 1:1325 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.![Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]](https://resources.bio-techne.com/images/products/Methyltransferase-like-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03290-img0011.jpg "Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]")
Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]
Immunocytochemistry/Immunofluorescence: Methyltransferase like 3 Antibody [NBP3-03290] - Immunofluorescence analysis of U-2 OS cells using Methyltransferase like 3 Rabbit pAb (NBP3-03290) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]](https://resources.bio-techne.com/images/products/Methyltransferase-like-3-Antibody-Immunohistochemistry-Paraffin-NBP3-03290-img0014.jpg "Immunohistochemistry-Paraffin: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]")
Immunohistochemistry-Paraffin: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]
Immunohistochemistry-Paraffin: Methyltransferase like 3 Antibody [NBP3-03290] - Mouse testis using METTL3 Rabbit pAb at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.![Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]](https://resources.bio-techne.com/images/products/Methyltransferase-like-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03290-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]")
![Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]](https://resources.bio-techne.com/images/products/Methyltransferase-like-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03290-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]")
![Immunohistochemistry-Paraffin: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]](https://resources.bio-techne.com/images/products/Methyltransferase-like-3-Antibody-Immunohistochemistry-Paraffin-NBP3-03290-img0013.jpg "Immunohistochemistry-Paraffin: Methyltransferase like 3 Antibody - Azide and BSA Free [NBP3-03290]")
Applications for Methyltransferase like 3 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Methyltransferase like 3
Alternate Names
adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase, EC 2.1.1.62, IME4, M6A, methyltransferase like 3, Methyltransferase-like protein 3, MGC4336, mRNA m(6)A methyltransferase, MTA70, MT-A70, N6-adenosine-methyltransferase 70 kDa subunit, Spo8
Gene Symbol
METTL3
Additional Methyltransferase like 3 Products
Product Documents for Methyltransferase like 3 Antibody - Azide and BSA Free
Product Specific Notices for Methyltransferase like 3 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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