METT10D Antibody (OTI3B5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72654

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Monkey, Primate

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3B5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all METT10D Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human METT10D(NP_076991) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

63.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for METT10D Antibody (OTI3B5) - Azide and BSA Free

Western Blot: METT10D Antibody (OTI3B5)Azide and BSA Free [NBP2-72654]

Western Blot: METT10D Antibody (OTI3B5)Azide and BSA Free [NBP2-72654]

Western Blot: METT10D Antibody (OTI3B5) - Azide and BSA Free [NBP2-72654] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY METT10D (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METT10D.
Immunohistochemistry: METT10D Antibody (OTI3B5) - Azide and BSA Free [NBP2-72654]

Immunohistochemistry: METT10D Antibody (OTI3B5) - Azide and BSA Free [NBP2-72654]

Immunohistochemistry: METT10D Antibody (OTI3B5) - Azide and BSA Free [NBP2-72654] - Staining of paraffin-embedded Human pancreas tissue using anti-METT10D mouse monoclonal antibody.
Flow Cytometry: METT10D Antibody (OTI3B5) - Azide and BSA Free [NBP2-72654]

Flow Cytometry: METT10D Antibody (OTI3B5) - Azide and BSA Free [NBP2-72654]

Flow Cytometry: METT10D Antibody (OTI3B5) - Azide and BSA Free [NBP2-72654] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY METT10D.
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Applications for METT10D Antibody (OTI3B5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: METT10D

Alternate Names

EC 2.1.1.-, methyltransferase 10 domain containing, Methyltransferase 10 domain-containing protein, methyltransferase like 16, METT10D, MGC3329, putative methyltransferase METT10D

Gene Symbol

METTL16

Additional METT10D Products

Product Documents for METT10D Antibody (OTI3B5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for METT10D Antibody (OTI3B5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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