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METT10D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35618

Novus Biologicals, part of Bio-Techne
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NBP3-35618-100ul
NBP3-35618-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 263-562 of human METT10D (NP_076991.3).

Sequence:
RIQGVPKVTYTEFCQGRTMRWALAWSFYDDVTVPSPPSKRRKLEKPRKPITFVVLASVMKELSLKASPLRSETAEGIVVVTTWIEKILTDLKVQHKRVPCGKEEVSLFLTAIENSWIHLRRKKRERVRQLREVPRAPEDVIQALEEKKPTPKESGNSQELARGPQERTPCGPALREGEAAAVEGPCPSQESLSQEENPEPTEDERSEEKGGVEVLESCQGSSNGAQDQEASEQFGSPVAERGKRLPGVAGQYLFKCLINVKKEVDDALVEMHWVEGQNRDLMNQLCTYIRNQIFRLVAVN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for METT10D Antibody

METT10D Antibody

Immunohistochemistry: METT10D Antibody [NBP3-35618] -

Immunohistochemistry: METT10D Antibody [NBP3-35618] - Immunohistochemistry analysis of paraffin-embedded Human spleen using METT10D Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
METT10D Antibody

Immunocytochemistry/ Immunofluorescence: METT10D Antibody [NBP3-35618] -

Immunocytochemistry/ Immunofluorescence: METT10D Antibody [NBP3-35618] - Immunofluorescence analysis of U-2 OS cells using METT10D Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
METT10D Antibody

Immunocytochemistry/ Immunofluorescence: METT10D Antibody [NBP3-35618] -

Immunocytochemistry/ Immunofluorescence: METT10D Antibody [NBP3-35618] - Immunofluorescence analysis of C6 cells using METT10D Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for METT10D Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Immunoprecipitation

0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: METT10D

Alternate Names

EC 2.1.1.-, methyltransferase 10 domain containing, Methyltransferase 10 domain-containing protein, methyltransferase like 16, METT10D, MGC3329, putative methyltransferase METT10D

Gene Symbol

METTL16

Additional METT10D Products

Product Documents for METT10D Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for METT10D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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