METTL14 Antibody (CL4254)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59044

Novus Biologicals, part of Bio-Techne
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NBP2-59044
NBP2-59044-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL4254

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all METTL14 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:RSWNMDSRLQEIRERQKLRRQLLAQQLGAESADSIGAVLNSKDEQREIAETRETCRASYDTSAPNAKRKYLDEGETDEDKMEEYKDELEMQQDEE

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for METTL14 Antibody (CL4254)

Western Blot: METTL14 Antibody (CL4254) [NBP2-59044]

Western Blot: METTL14 Antibody (CL4254) [NBP2-59044]

Western Blot: METTL14 Antibody (CL4254) [NBP2-59044] - Analysis in human cell line A-431.
Immunocytochemistry/ Immunofluorescence: METTL14 Antibody (CL4254) [NBP2-59044]

Immunocytochemistry/ Immunofluorescence: METTL14 Antibody (CL4254) [NBP2-59044]

Immunocytochemistry/Immunofluorescence: METTL14 Antibody (CL4254) [NBP2-59044] - Staining of RH-30 cells using the Anti-METTL14 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: METTL14 Antibody (CL4254) [NBP2-59044]

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4254) [NBP2-59044]

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4254) [NBP2-59044] - Staining of human duodenum shows strong nuclear immunoreactivity in glandular cells and in the connective tissue.
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Applications for METTL14 Antibody (CL4254)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: METTL14

Probable methyltransferase

Long Name

Methyltransferase-Like Protein 14

Alternate Names

KIAA1627

Gene Symbol

METTL14

Additional METTL14 Products

Product Documents for METTL14 Antibody (CL4254)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for METTL14 Antibody (CL4254)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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