MGAT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52077

Novus Biologicals, part of Bio-Techne
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NBP1-52077
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all N-Acetylglucosaminyltransferase I/MGAT1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QVEKVRTNDR corresponding to internal region according to NP_002397.2.

Epitope

C-QVEKVRTNDR

Specificity

Reported variants represent identical protein: NP_002397.2, NP_001108090.1, NP_001108091.1, NP_001108092.1, NP_001108089.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MGAT1 Antibody

Western Blot: MGAT1 Antibody [NBP1-52077]

Western Blot: MGAT1 Antibody [NBP1-52077]

Western Blot: MGAT1 Antibody [NBP1-52077] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MGAT1 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B.

Applications for MGAT1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.2 - 1 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing full-length human MGAT1 (myc and DYKDDDDK tagged), a band of approx. 55 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: N-Acetylglucosaminyltransferase I/MGAT1

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene.

Long Name

Mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase

Alternate Names

GGNT1, GlcNac-TI, GLCT1, GNT-1

Entrez Gene IDs

4245 (Human); 17308 (Mouse); 81519 (Rat)

Gene Symbol

MGAT1

UniProt

NP_002397.2 (Human)

Additional N-Acetylglucosaminyltransferase I/MGAT1 Products

Product Documents for MGAT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MGAT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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