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MGAT4A Antibody (4H4)

Novus Biologicals, part of Bio-Techne | Catalog # H00011320-M02

Novus Biologicals, part of Bio-Techne
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H00011320-M02

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4H4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MGAT4A (NP_036346, 436 a.a. ~ 535 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PVNVESYLFHSGNQEHPGDILLNTTVEVLPFKSEGLEISKETKDKRLEDGYFRIGKFENGVAEGMVDPSLNPISAFRLSVIQNSAVWAILNEIHIKKATN

Reactivity Notes

Other species not tested. Rat reactivity reported in scientific literature (PMID: 26104473)

Specificity

MGAT4A - mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MGAT4A Antibody (4H4)

Western Blot: MGAT4A Antibody (4H4) [H00011320-M02]

Western Blot: MGAT4A Antibody (4H4) [H00011320-M02]

Western Blot: MGAT4A Antibody (4H4) [H00011320-M02] - MGAT4A monoclonal antibody (M02), clone 4H4 Analysis of MGAT4A expression in Jurkat.
Immunohistochemistry-Paraffin: MGAT4A Antibody (4H4) [H00011320-M02]

Immunohistochemistry-Paraffin: MGAT4A Antibody (4H4) [H00011320-M02]

Immunohistochemistry-Paraffin: MGAT4A Antibody (4H4) [H00011320-M02] - Analysis of monoclonal antibody to MGAT4A on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 0.2 ug/ml.
Sandwich ELISA: MGAT4A Antibody (4H4) [H00011320-M02] - Detection limit for recombinant GST tagged MGAT4A is approximately 0.1ng/ml as a capture antibody.

Applications for MGAT4A Antibody (4H4)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MGAT4A

This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation.

Long Name

alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A

Alternate Names

EC 2.4.1.145, GlcNAc-T IVa, GnT-4a, GNT-IV, GNT-IVA, N-Acetylglucosaminyltransferase IVa

Entrez Gene IDs

11320 (Human)

Gene Symbol

MGAT4A

OMIM

604623 (Human)

Additional MGAT4A Products

Product Documents for MGAT4A Antibody (4H4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MGAT4A Antibody (4H4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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