MgcRacGAP/RACGAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47374

Novus Biologicals, part of Bio-Techne
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NBP2-47374
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MgcRacGAP Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SALAFLNRGQPSSSNAGNKRLSTIDESGSILSDISFDKTDESLDWDSSLVKTFKLKKREKRRSTSRQFVDGPPGPVKKTRSIGSAVDQGNESIVAKTTVTVPNDGGPIEAVSTIET

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MgcRacGAP/RACGAP1 Antibody

Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP2-47374]

Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP2-47374]

Western Blot: MgcRacGAP/RACGAP1 Antibody [NBP2-47374] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-RACGAP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: MgcRacGAP/RACGAP1 Antibody [NBP2-47374]

Immunocytochemistry/ Immunofluorescence: MgcRacGAP/RACGAP1 Antibody [NBP2-47374]

Immunocytochemistry/Immunofluorescence: MgcRacGAP/RACGAP1 Antibody [NBP2-47374] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP2-47374]

Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP2-47374]

Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody [NBP2-47374] - Staining of human Placenta shows strong nuclear positivity in trophoblastic cells.
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Applications for MgcRacGAP/RACGAP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1:100 - 1:250
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MgcRacGAP

Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.[supplied by OMIM]

Long Name

Male Germ Cell Rac GTPase-activating Protein

Alternate Names

RACGAP1

Gene Symbol

RACGAP1

Additional MgcRacGAP Products

Product Documents for MgcRacGAP/RACGAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MgcRacGAP/RACGAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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