mGluR5 Antibody (ST51-00)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11866

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP3-11866-100ul
NBP3-11866-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ST51-00

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all mGluR5 Antibodies »

Product Summary for mGluR5 Antibody (ST51-00)

Immunogen

Recombinant protein (Uniprot#: P41594)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

132 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for mGluR5 Antibody (ST51-00)

Immunocytochemistry/ Immunofluorescence: mGluR5 Antibody (ST51-00) [NBP3-11866]

Immunocytochemistry/ Immunofluorescence: mGluR5 Antibody (ST51-00) [NBP3-11866]

Immunocytochemistry/Immunofluorescence: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunocytochemistry staining mGluR5 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: mGluR5 Antibody (ST51-00) [NBP3-11866]

Immunohistochemistry-Paraffin: mGluR5 Antibody (ST51-00) [NBP3-11866]

Immunohistochemistry-Paraffin: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using NBP3-11866. Counter stained with hematoxylin.
Flow Cytometry: mGluR5 Antibody (ST51-00) [NBP3-11866]

Flow Cytometry: mGluR5 Antibody (ST51-00) [NBP3-11866]

Flow Cytometry: mGluR5 Antibody (ST51-00) [NBP3-11866] - Flow cytometric analysis of SH-SY-5Y cells with NBP3-11866 at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Applications for mGluR5 Antibody (ST51-00)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:200-1:500

Western Blot

1:1000 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1X TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: mGluR5

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Class I metabotropic glutamate receptors (mGluRs) have been postulated to play a role in synaptic plasticity. It has been suggested that mGluR5 may be the primary high affinity ACPD receptor in CA1 neurons (1). Also, neuroprotection by mGluR system has been linked to the modulation of both the free radical nitric oxide (NO) and programmed cell death (PCD). Activation of group I mGluR subtypes utilized an effective, "upstream" mechanism for the inhibition of cysteine protease activity that offered an enhanced level of neuroprotection through both the preservation of genomic DNA integrity and the maintenance of PS membrane asymmetry (2). It has also been shown that activation of mGluRs enhances formation of an mGluR-Homer-PIKE-L complex, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Our findings indicate that this complex mediates the well-known ability of agonists of mGluR5 to prevent neuronal apoptosis (3).

Long Name

Metabotropic Glutamate Receptor 5

Alternate Names

GPRC1E, GRM5, mGlu5

Gene Symbol

GRM5

Additional mGluR5 Products

Product Documents for mGluR5 Antibody (ST51-00)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for mGluR5 Antibody (ST51-00)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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