MGMT Antibody (2A4X3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15348

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15348-100ul
NBP3-15348-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2A4X3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MGMT Antibodies »

Product Summary for MGMT Antibody (2A4X3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 108-207 of human MGMT (NP_002403.3). FGEVISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHRLGKPGLGGSSGLAGAWLKGAGATSGSPPAGRN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MGMT Antibody (2A4X3)

Western Blot: MGMT Antibody (2A4X3) [NBP3-15348]

Western Blot: MGMT Antibody (2A4X3) [NBP3-15348]

Western Blot: MGMT Antibody (2A4X3) [NBP3-15348] - Western blot analysis of extracts of various cell lines, using MGMT Rabbit mAb (NBP3-15348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for MGMT Antibody (2A4X3)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MGMT

O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytotoxic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.

MGMT antibodes are useful tools for DNA repair studies and for some cancer research.

Long Name

O-6 Methylguanine-DNA Methyltransferase

Alternate Names

6-O-methylguanine-DNA methyltransferase, EC 2.1.1.63, methylated-DNA--protein-cysteine methyltransferase, methylguanine-DNA methyltransferase, O-6-methylguanine-DNA methyltransferase, O6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase

Gene Symbol

MGMT

Additional MGMT Products

Product Documents for MGMT Antibody (2A4X3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MGMT Antibody (2A4X3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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