MGMT Antibody (MGMT/8128R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20454
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Kappa Clone # MGMT/8128R
Concentration
0.2 mg/ml
Product Summary for MGMT Antibody (MGMT/8128R)
Immunogen
Recombinant fragment (around aa1-100) of human MGMT (exact sequence is proprietary)
Localization
Nucleus
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: Jurkat or MCF-7 cells. Human tonsil or stomach (IHC).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for MGMT Antibody (MGMT/8128R)
MGMT Antibody (MGMT/8128R)
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using MGMT antibody (MGMT/8128R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
MGMT Antibody (MGMT/8128R)
Formalin-fixed, paraffin-embedded human stomach (corpus) stained with MGMT antibody (MGMT/8128R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
MGMT Antibody (MGMT/8128R)
Formalin-fixed, paraffin-embedded human parotid gland stained with MGMT antibody (MGMT/8128R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for MGMT Antibody (MGMT/8128R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MGMT
MGMT antibodes are useful tools for DNA repair studies and for some cancer research.
Long Name
O-6 Methylguanine-DNA Methyltransferase
Alternate Names
6-O-methylguanine-DNA methyltransferase, EC 2.1.1.63, methylated-DNA--protein-cysteine methyltransferase, methylguanine-DNA methyltransferase, O-6-methylguanine-DNA methyltransferase, O6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase
Gene Symbol
MGMT
Additional MGMT Products
Product Documents for MGMT Antibody (MGMT/8128R)
Product Specific Notices for MGMT Antibody (MGMT/8128R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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