MGMT Antibody (S01-8B1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19480

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S01-8B1

Concentration

0.3 mg/ml

View all MGMT Antibodies »

Product Summary for MGMT Antibody (S01-8B1)

Immunogen

A synthetic peptide of human MGMT (Uniprot # P16455)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MGMT Antibody (S01-8B1)

Western Blot: MGMT Antibody (S01-8B1) [NBP3-19480]

Western Blot: MGMT Antibody (S01-8B1) [NBP3-19480]

Western Blot: MGMT Antibody (S01-8B1) [NBP3-19480] - Western blot detection of MGMT in Jurkat cell lysates using NBP3-19480 (1:1000 diluted). Predicted band size: 22kDa. Observed band size: 22kDa.
Immunohistochemistry-Paraffin: MGMT Antibody (S01-8B1) [NBP3-19480]

Immunohistochemistry-Paraffin: MGMT Antibody (S01-8B1) [NBP3-19480]

Immunohistochemistry-Paraffin: MGMT Antibody (S01-8B1) [NBP3-19480] - Immunohistochemistry of MGMT in paraffin-embedded Human colon cancer tissue using NBP3-19480 at dilution 1/100

Applications for MGMT Antibody (S01-8B1)

Application
Recommended Usage

Immunohistochemistry

1/50-1/200

Western Blot

1/1000-1/5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MGMT

O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytotoxic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.

MGMT antibodes are useful tools for DNA repair studies and for some cancer research.

Long Name

O-6 Methylguanine-DNA Methyltransferase

Alternate Names

6-O-methylguanine-DNA methyltransferase, EC 2.1.1.63, methylated-DNA--protein-cysteine methyltransferase, methylguanine-DNA methyltransferase, O-6-methylguanine-DNA methyltransferase, O6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase

Gene Symbol

MGMT

Additional MGMT Products

Product Documents for MGMT Antibody (S01-8B1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MGMT Antibody (S01-8B1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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